Quenching effect on gold nano-patterned cardiac troponin I chip by total internal reflection fluorescence microscopy

► Quenching effects on gold nano-patterned chips with a spot diameter of 500 nm. ► Quantitative analysis of cTnI using the prism- and objective-type TIRFM. ► Stronger quenching efficiency in the prism-than in the objective-type TIRFM. ► Improved detection sensitivity of cTnI at the aM concentration level.