In-capillary formation of polymer/surfactant complexes-assisted reversed-migration micellar electrokinetic chromatography for facile analysis of neutral steroids

► An IPSC-assisted RM-MEKC for neutral steroid analysis is proposed. ► Poly(N-isopropylacrylamide) and SDS are used as pseudostationary phases. ► The polymer size, concentrations, end-group charges, and SDS concentrations are evaluated.