Determination of glibenclamide and puerarin in rat plasma by UPLC-MS/MS: Application to their pharmacokinetic interaction study

► Drug-herb interaction is a significant safety concern. ► A method was developed to determine glibenclamide and puerarin in rat plasma. ► Pharmacokinetic interaction between glibenclamide and puerarin was studied. ► Pueraria lobata extract could slow down the metabolism of glibenclamide. ► Glibenclamide could accelerate the absorption of puerarin.