Enzymatic analysis of α-ketoglutaramate-A biomarker for hyperammonemia

► New enzymatic assays were developed for the analysis of α-ketoglutaramate (KGM). ► The analysis demonstrated robustness operating in human serum solutions. ► The analysis discriminated normal and pathophysiological concentrations of KGM. ► Future clinical application of the KGM analysis in biological fluids is feasible.