Determination of Zn-citrate in human milk by CIM monolithic chromatography with atomic and mass spectrometry detection

► Reported is new analytical procedure for quantitative determination of Zn-citrate in human milk. ► LMM-Zn fraction was separated from milk proteins by microultrafiltration. ► CIM monolithic chromatography enabled excellent separation of Zn-citrate complexes. ► FAAS or ICP-MS were employed for quantification Zn-citrate. ► Zn-binding ligand was identified by ESI-MS-MS.