Rapid sample pre-treatment prior to GC-MS and GC-MS/MS urinary toxicological screening

► Enzymatic hydrolysis of glucuro-conjugates in urine can be performed in 5 min thanks to microwaves. ► The use of a deconvolution software allowed for the reduction of the GC run to 10 min. ► GC-MS/MS appears as potential alternative to immunoassays for drug screening.