Detection of food-borne pathogens with DNA arrays on disk

► First work, describing a DNA array for duplex detection of food-borne pathogens on a DVD using compact disk technology. ► The compact disk laboratory equipment is capable of detecting microorganisms contaminants at low cfu/mL of milk samples. ► The methodology is portable, cheap and rapid and accomplishes tens of samples simultaneously within a disc. ► Results were in good agreement with those obtained by the reference quantitative real-time PCR method. ► Detection of DNA from pathogens with potential applications in clinic, food safety and quality assurance.