Automated flow-through amperometric immunosensor for highly sensitive and on-line detection of okadaic acid in mussel sample

► In this study, we developed a fully automated flow based immunosensor for OA detection. ► The system was fabricated by injecting modified MBs into the flow cell as solid support to immobilize OA. ► An indirect competitive immunoassay format under automated flow conditions was performed for OA analysis. ► The developed flow immunosensor increased the sensitivity of system as compared to batch systems. ► The developed immunosensor was validated with mussel sample.