A simple way to configure on-line two-dimensional liquid chromatography for complex sample analysis: Acquisition of four-dimensional data

► Set up HPLC×UPLC-TOF MS based on commercial chromatographs. ► 4D data containing 2D retention times, intensity and m/z ratios. ► As many as 398 peaks detected from a complex sample, TCM Qingkailing. ► Compared with HPLC×HPLC, reduce the analysis time by 75%. ► Increase the number of recognized components by three times.