Preparation of molecular imprinted polymers using bi-functional monomer and bi-crosslinker for solid-phase extraction of rutin

► The preparation of rutin molecularly imprinted polymer for the extracts of real samples. ► Using bi-functional monomer and bi-cross-linker. ► Functional monomer and cross-linker and the molar ratio were optimized, respectively. ► The MIPs were characterized by FTIR and SEM and different adsorption experiments. ► MISPE protocol was optimized for mixed standard solutions and extracts of real samples.