Urine iodide determination by ion-pair reversed-phase high performance liquid chromatography and pulsed amperometric detection

► We developed a HPLC-PAD method for determining iodide concentration in urine. ► Pulsed amperometric detection improves peak shape and electrode stability. ► Two extraction steps for sample purification are necessary. ► This method is sensitive, selective, precise and accurate. ► The method is effective for urinary iodine determination.