Multiclass analysis of antibacterial residues in milk using RP-liquid chromatography with photodiode array and fluorescence detection and tandem mass spectrometer confirmation

► Sulfonamides, fluoroquinolones and ceftiofur were determined in milk. ► Acidified water was efficient for extraction without further clean up procedure. ► Validation results showed great performance for the LC method. ► Analyzed samples from Brazil showed no detection. ► A feasible alternative to monitor residues in milk was developed.