Determination of pravastatin and pravastatin lactone in rat plasma and urine using UHPLC-MS/MS and microextraction by packed sorbent

► Determination of pravastatin and pravastatin lactone in rat plasma and urine. ► MEPS-UHPLC-MS/MS in ESI polarity-switching mode uses of deuterium labeled standards. ► MEPS as fast and simple sample preparation using small sample volume. ► Application to real plasma and urine sample to evaluate pharmacokinetic profiles.