Development and validation of UHPLC-ESI-MS/MS method for the determination of selected cardiovascular drugs, polyphenols and their metabolites in human urine

► Development of new UHPLC-ESI-MS/MS method for the determination of cardiovascular drugs, polyphenols and their metabolites. ► Optimisation the MS/MS and chromatographic conditions. ► Separation the 52 compounds in less than 10 min. ► Application developed method to the detection and quantitation of the studied analytes in urine samples.