Pharmacokinetics and metabolite identification of a novel VEGFR-2 and Src dual inhibitor 6-chloro-2-methoxy-N-(2-methoxybenzyl) acridin-9-amine in rats by liquid chromatography tandem mass spectrometry

► A robust LC-MS/MS method was developed for MBAA analysis in rat body fluid samples. ► Main pharmacokinetics parameters of MBAA were measured. ► Metabolic pathways of MBAA were presumed in the liver of rats. ► MBAA was suggested to metabolism and a strong first-pass clearance in the liver.