Optimization of the analytical extraction of polyamines from milk

► Polyamines are growth promoters and modulate intestinal tract and immune response. ► A method for amines in milk and in milk products was optimized and validated. ► Optimum extraction of polyamines - 40 s vortexing, 4 extractions, and SSA at 1.5%. ► Milk and beverages are poor sources of polyamines, human milk contains both amines.