کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7691020 | 1495972 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ATR-FTIR analysis of spectral and biochemical changes in glioma cells induced by chlorotoxin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: ATR-FTIR analysis of spectral and biochemical changes in glioma cells induced by chlorotoxin ATR-FTIR analysis of spectral and biochemical changes in glioma cells induced by chlorotoxin](/preview/png/7691020.png)
چکیده انگلیسی
Chlorotoxin (CTX) is a small peptide with unique targeting properties towards gliomas and other tumors of the neuroectodermal origin. Despite being investigated in many in vivo and in vitro targeted drug delivery and imaging studies, the mechanism of interaction between CTX and tumor cells has not been well understood. This study presents a new approach in monitoring the biochemical and biophysical changes in glioma cells after being exposed to CTX using Attenuated Total Reflection Fourier Transform Infra-Red (ATR-FTIR) spectroscopy. In the first part, we characterized the signature spectra of CTX and U87 cells. Next, we evaluated the differences in biochemical compositions of the spectra of the glioma cells treated with and without CTX over different incubation time periods. Our results indicated biochemical changes in U87 cells at different stages of incubation with CTX, with the most notable changes occurring after 30Â min of incubation. Detailed comparisons of the spectra of the U87 cells treated with and without CTX for 30Â min revealed distinct changes and shifts of the bands associated with proteins and lipids. In particular, a downshift of the band assigned to CH2 scissoring of lipid membranes from 1454Â cmâ1 to 1448Â cmâ1 followed by an appearance of a new shoulder at wavenumber of 1465Â cmâ1 revealed conformational changes in hydrocarbon chain packing in lipid bilayers of U87 cells due to CTX. Other band shifts indicated additional changes in conformation and orientation of lipids and proteins in U87 cells exposed to CTX.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 87, November 2016, Pages 164-172
Journal: Vibrational Spectroscopy - Volume 87, November 2016, Pages 164-172
نویسندگان
Rana Falahat, Marzenna Wiranowska, Ryan Toomey, Norma Alcantar,