کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7692092 | 1496264 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Eicosapentaenoic acid and docosahexaenoic acid have distinct membrane locations and lipid interactions as determined by X-ray diffraction
ترجمه فارسی عنوان
اسید ایکوزاپنتانوئیک و اسید داکوزاگزنئوئیک دارای مکان های غشایی متمایز و تعامل لیپید با تعیین پراش اشعه ایکس
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کلمات کلیدی
اسیدهای چرب امگا 3، پراش اشعه ایکس، ساختار غشاء،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
چکیده انگلیسی
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) differentially influence lipid oxidation, signal transduction, fluidity, and cholesterol domain formation, potentially due in part to distinct membrane interactions. We used small angle X-ray diffraction to evaluate the EPA and DHA effects on membrane structure. Membrane vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol (C) (0.3C:POPC mole ratio) were prepared and treated with vehicle, EPA, or DHA (1:10â¯mol ratio to POPC). Electron density profiles generated from the diffraction data showed that EPA increased membrane hydrocarbon core electron density over a broad area, up toâ¯Â±â¯20â¯Ã
from the membrane center, indicating an energetically favorable extended orientation for EPA likely stabilized by van der Waals interactions. By contrast, DHA increased electron density in the phospholipid head group region starting atâ¯Â±â¯12â¯Ã
from the membrane center, presumably due to DHA-surface interactions, with coincident reduction in electron density in the membrane hydrocarbon core centeredâ¯Â±â¯7-9â¯Ã
from the membrane center. The membrane width (d-space) decreased by 5â¯Ã
in the presence of vehicle as the temperature increased from 10â¯Â°C to 30â¯Â°C due to increased acyl chain trans-gauche isomerizations, which was unaffected by addition of EPA or DHA. The influence of DHA on membrane structure was modulated by temperature changes while the interactions of EPA were unaffected. The contrasting EPA and DHA effects on membrane structure indicate distinct molecular locations and orientations that may contribute to observed differences in biological activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 212, May 2018, Pages 73-79
Journal: Chemistry and Physics of Lipids - Volume 212, May 2018, Pages 73-79
نویسندگان
Samuel C.R. Sherratt, R. Preston Mason,