کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7692383 1496297 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biosensor based on lectin and lipid membranes for detection of serum glycoproteins in infected patients with dengue
ترجمه فارسی عنوان
بیوسنسور بر اساس غلظت های لکتین و چربی برای تشخیص گلیکوپروتئین های سرم در بیماران مبتلا به دیابت
کلمات کلیدی
دنگ لکتین، فسفولیپید، بیوسنسور، گلیکوپروتئین ها،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی
In this work, we developed a biosystem based on Concanavalin A (ConA) and lipid membranes to recognize glycoproteins from the serum of patients contaminated with dengue serotypes 1, 2 and 3 (DENV1, DENV2 and DENV3). The modified gold electrode was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy. Morphological analyses of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC-ConA, DPPC-ConA-DENV1, DPPC-ConA-DENV2 and DPPC-ConA-DENV3 revealed the existence of a non-uniform covering and large globules. EIS and CV measurements have shown that redox probe reactions on the modified gold electrodes were partially blocked due to the adsorption of lipid-ConA system and reveal the interaction response of the immobilized ConA to the presence of glycoproteins of dengue serum. The biosystem exhibited a wide linear response to different concentrations of sera of dengue serotypes 1, 2 and 3. A higher impedimetric response to glycoproteins present in dengue serotype 3 was observed. Our results demonstrate the applicability of lectin and lipid membranes to the development of biosensors for dengue infections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 180, May 2014, Pages 7-14
نویسندگان
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