کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7692952 | 1496323 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New pH-sensitive liposomes containing phosphatidylethanolamine and a bacterial dirhamnolipid
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
RhamnolipidsN-(lissamine rhodamine B sulfonyl)phosphatidylethanolaminePoPCMLVN-NBD-PEWAXSSAXS1,2-dioleoyl-sn-glycero-3-phosphoethanolamine - 1،2-dioleoyl-sn-glycero-3-phosphoethanolaminemultilamellar vesicles - vesicles multilamellarwide angle X-ray scattering - اشعه ایکس اشعه ایکس پراکندگیlarge unilamellar vesicles - بزرگ کیسه های بدون انعطاف پذیرBiosurfactants - بیوسورفکتانت هاPseudomonas aeruginosa - سودوموناس آئروژینوزاPhospholipid membranes - غشای فسفولیپیدLUV - لووpH-sensitive liposomes - لیپوزوم های حساس به pHsmall angle X-ray Scattering - پراکندگی اشعه ایکس زاویه کوچکDOPE - پیش بینی کردن
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Phosphatidylethanolamine-based pH-sensitive liposomes of various compositions have been described as efficient systems for cytoplasmic delivery of molecules into cells. Incorporation of an amphiphile of appropriate structure is needed for the stabilization and performance of these vesicles. Among the wide variety of interesting activities displayed by Pseudomonas aeruginosa dirhamnolipids (diRL), is their capacity to stabilize bilayer structures in phosphatidylethanolamine systems. In this work, X-ray scattering, dynamic light scattering, fluorescence spectroscopy and fluorescence microscopy have been used to study the structure and pH-dependent behaviour of phosphatidylethanolamine/diRL liposomes. We show that diRL, in combination with dioleoylphosphatidylethanolamine (DOPE), forms stable multilamellar and unilamellar liposomes. Acidification of DOPE/diRL vesicles leads to membrane destabilization, fusion, and release of entrapped aqueous vesicle contents. Finally, DOPE/diRL pH-sensitive liposomes act as efficient vehicles for the cytoplasmic delivery of fluorescent probes into cultured cells. It is concluded that DOPE/diRL form stable pH-sensitive liposomes, and that these liposomes are incorporated into cultured cells through the endocytic pathway, delivering its contents into the cytoplasm, which means a potential use of these liposomes for the delivery of foreign substances into living cells. Our results establish a new application of diRL as a bilayer stabilizer in phospholipid vesicles, and the use of diRL-containing pH-sensitive liposomes as delivery vehicles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 164, Issue 1, January 2011, Pages 16-23
Journal: Chemistry and Physics of Lipids - Volume 164, Issue 1, January 2011, Pages 16-23
نویسندگان
Marina Sánchez, Francisco J. Aranda, José A. Teruel, Antonio Ortiz,