کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7704848 1645386 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bienzyme reactions on cross-linked DNA scaffolds for electrochemical analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Bienzyme reactions on cross-linked DNA scaffolds for electrochemical analysis
چکیده انگلیسی
Enzymes play an essential role in various detection technologies. We show here that interstrand cross-linked oligodeoxynucleotides (CL-ODNs) can provide stable scaffolds for efficiently coupling two types of enzymatic reactions on an electrode. Glucose can be electrochemically detected using glucose oxidase (GOx) and horseradish peroxidase (HRP). When both GOx and HRP were immobilized on an electrode surface by attachment at the termini of CL-ODNs, the current value was markedly increased compared with that obtained on a standard ODN scaffold. The relative orientation of the enzymes on the electrode strongly affected the current intensities. The CL-ODN also allowed GOx-HRP to form a complex on the tiny surface of a microelectrode, resulting in the imaging of local glucose distribution. These results suggest that CL-ODNs have potential utility in other sensing technologies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 113, February 2017, Pages 15-19
نویسندگان
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