کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7706970 1497314 2018 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of the nitrogen source on the hydrogen production metabolism and hydrogenases of Clostridium butyricum CWBI1009
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Effect of the nitrogen source on the hydrogen production metabolism and hydrogenases of Clostridium butyricum CWBI1009
چکیده انگلیسی
Investigations were carried out to determine the effect of various concentrations of organic and ammonium nitrogen sources on fermentative hydrogen production by the strain Clostridium butyricum CWBI1009. The results indicated that the H2-producing metabolism of the strain is favoured within the range (0.56-0.062 gN/L) of peptone and (NH4)2SO4. Optimal overall performance (i.e. 1.43 ± 0.08 mol H2/mol glucose and 1.08 ± 0.03 mLH2/h, respectively) was achieved with 0.062 gN/L of casein peptone. The study of the amino acid uptake and the gene expression pattern for four [FeFe]-hydrogenases and the nitrogenase showed that nitrogen was in excess in all the experiments with a nitrogen concentration above 0.062 gN/L and, at that optimal concentration, the expression of the HydB2 gene would be responsible for the much higher H2 yield.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 43, Issue 11, 15 March 2018, Pages 5451-5462
نویسندگان
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