Process parameter optimization and enhancement of photo-biohydrogen production by mixed culture of Rhodobacter sphaeroides NMBL-02 and Escherichia coli NMBL-04 using Fe-nanoparticle

Response surface methodology analysis showed best fitting of experimental data with statistical model. At the optimal conditions, the cumulative hydrogen production was reported as 2046 mL H2/L of medium which was in vicinity of predicted value (2029.405 mL H2/L).