Double-chamber microbial fuel cells started up under room and low temperatures

This study examined the performances of two double-chamber microbial fuel cells (MFCs) at 25 °C and 15 °C. After successful startup, the cell temperature of MFC A was decreased from 25 to 15 °C, yielding a sudden breakdown of the entire system. Conversely, the MFC B, started up at 15 °C, delivering higher power density at 25 °C than MFC A at the same temperature. The electrochemical analysis revealed that the MFC B had lower anodic resistance than MFC A. Additionally, a negative temperature dependence of the polarization resistances of the anodic biofilm was noted, a novel phenomenon only reported in this double-chambered study. Microbial analysis showed that the psychrophilic bacteria were enriched in anodic biofilms of MFC B, which likely contributed to the robust cell performance of the present double-chambered MFCs.