A novel screening method for hydrogenase-deficient mutants in Chlamydomonas reinhardtii based on in vivo chlorophyll fluorescence and photosystem II quantum yield

► In Chlamydomonas, PSII efficiency in anaerobiosis is hydrogenase-dependant. ► Chlorophyll fluorescence signature allows screening hydrogenase-deficient mutants. ► The developed screening strategy is fast, sensitive and non-invasive. ► HydG-2 mutant behaves the same as HydEF-1 mutant regarding photosynthesis.