کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7786781 | 1500622 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anticoagulant and antithrombotic activities of modified xylofucan sulfate from the brown alga Punctaria plantaginea
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Selectively and totally sulfated (1 â 3)-linked linear homofucans bearing â¼20 monosaccharide residues on average have been prepared from the branched xylofucan sulfate isolated from the brown alga Punctaria plantaginea. Anticoagulant and antithrombotic properties of the parent biopolymer and its derivatives were assessed in vitro. Highly sulfated linear fucan derivatives were shown to inhibit clot formation in APTT assay and ristocetin induced platelets aggregation, while the partially sulfated analogs were inactive. In the experiments with purified proteins, fucan derivatives with degree of sulfation of â¼2.0 were found to enhance thrombin and factor Xa inhibition by antithrombin III. The effect of sulfated fucans on thrombin inhibition, which was similar to those of heparinoid Clexane® (enoxaparin) and of a fucoidan from the brown alga Saccharina latissima studied previously, can be explained by the multicenter interaction and formation of a ternary complex thrombin-antithrombin III-polysaccharide. The possibility of such complexation was confirmed by computer docking study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 136, 20 January 2016, Pages 826-833
Journal: Carbohydrate Polymers - Volume 136, 20 January 2016, Pages 826-833
نویسندگان
Nadezhda E. Ustyuzhanina, Maria I. Bilan, Alexey G. Gerbst, Natalia A. Ushakova, Eugenia A. Tsvetkova, Andrey S. Dmitrenok, Anatolii I. Usov, Nikolay E. Nifantiev,