کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7787516 1500625 2015 42 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants
ترجمه فارسی عنوان
خواص آماده سازی و خواص زیست محیطی پوششهای کامپوزیت کیتوزان و کازئین فسفوپتید برای ایمپلنت های ارتوپدی
کلمات کلیدی
آلومینیوم کروم کروم، لایه لایه، کیتوزان، فوزوپپتید های کازئین، فعالیت زیستی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی
Using the layer-by-layer deposition method, functional chitosan/casein phospopeptides (CS/CPP) composite coatings were produced on Co-Cr-Mo alloy. The CS/CPP composite coatings had the dendritic topography, and were quite hydrophilic. Zeta potential measurements showed the composite coatings were negative charged at neural pH. XPS results indicated that the CS/CPP composite coatings were covalently bond to the substrate. When MC3T3-E1 cells were seeded on the CS/CPP composite coatings, no cytotoxicity was observed. The bone morphogenetic protein-2 (BMP-2) mRNA expression was significantly up-regulated in MC3T3-E1 cells cultured on the composite coatings and it was twice as much as that of cells cultured on the bare substrate. The expression of osteoprotegerin (OPG) mRNA and the ratio of OPG/receptor activator of nuclear factor-κB ligand (RNAKL) mRNA were increased 5-fold and 55-fold, respectively. These results suggested the CS/CPP composite coatings may have potential application in cobalt matrix orthopaedic implants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 133, 20 November 2015, Pages 236-244
نویسندگان
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