کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7787704 | 1500627 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alginate as a protease inhibitor in vitro and in a model gut system; selective inhibition of pepsin but not trypsin
ترجمه فارسی عنوان
آلژینات به عنوان بازدارنده پروتئاز در محیط آزمایشگاهی و در یک مدل سیستم گوارش؛ مهار باز انتخاب پپسین، اما نه تریپسین
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کلمات کلیدی
آلژینات، پپسین، تریپسین، پروتئولیز، سیستم گوارش مدل،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
Alginates are widely used in the food and medical industries, including as a Gastro-Oesophagul Reflux treatment. This work investigates the inhibitory effects of alginate on the reflux aggressors trypsin and pepsin and the role of alginate-substrate binding, pH and alginate structure on inhibition. Alginates were shown to reduce pepsin activity by up to 53.9% (±9.5SD) in vitro. Strong positive correlation between alginate mannuronate residue frequency and levels of pepsin inhibition was observed. Limited inhibition of trypsin was shown. Viscometric observations of pH dependent interactions between alginate and protein suggest a mechanism whereby pH dependent ionic interactions reduce substrate availability to enzyme at acidic pH. To understand how dietary protein digestion is affected by alginate, proteolytic digestion was investigated in an in vitro model of the upper digestive tract. Significant inhibition of proteolysis was shown in the gastric phase of digestion, but not the small intestinal phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 131, 20 October 2015, Pages 142-151
Journal: Carbohydrate Polymers - Volume 131, 20 October 2015, Pages 142-151
نویسندگان
Peter Ian Chater, Mathew D. Wilcox, Iain A. Brownlee, Jeffrey P. Pearson,