کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7791330 | 1500646 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosynthesis of oligodextrans with different Mw by synergistic catalysis of dextransucrase and dextranase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biosynthesis of oligodextrans with different Mw by synergistic catalysis of dextransucrase and dextranase Biosynthesis of oligodextrans with different Mw by synergistic catalysis of dextransucrase and dextranase](/preview/png/7791330.png)
چکیده انگلیسی
In this study, we have investigated various adding times of dextranase to the dextransucrase system to reveal the synergistic process of dextransucrase and dextranase. Dextranase added into the dextransucrase-sucrose system at different times gave rise to different main dextran products. The results showed that dextranase added into sucrose system at the same time with dextransucrase synthesized low molecular weight (Mw) dextran targeted to 5 kDa, while dextranase added during the reaction process of dextransucrase directionally prepared dextran with medium Mw of 10 kDa and 20 kDa. Fourier-transform infrared (FTIR) spectroscopy and 1H Nuclear magnetic resonance (NMR) exhibited that the synthesized oligodextrans were mainly composed of α-1,6-glycosidic linkages (dextran 6587 Da, 96.82%; dextran 22,521 Da, 96.03%) and low α-1,3-glycosidic branch. The research established the relationship between the synergistic catalysis of the double enzymatic system and the synthesis of oligodextrans with different Mw.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 112, 4 November 2014, Pages 387-395
Journal: Carbohydrate Polymers - Volume 112, 4 November 2014, Pages 387-395
نویسندگان
Weiwei Gan, Hongbin Zhang, Yuqi Zhang, XueQin Hu,