کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7792554 1500655 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biochemical and atomic force microscopic characterization of salmon nasal cartilage proteoglycan
ترجمه فارسی عنوان
مشخصه میکروسکوپ نیروی بیوشیمیایی و اتمی پروتئول گلیس غضروف بینی سالمون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی
Biological activities of salmon nasal cartilage proteoglycan fractions are known, however, structural information is lacking. Recently, the major proteoglycan of this cartilage was identified as aggrecan. In this study, global molecular images and glycosaminoglycan structure of salmon nasal cartilage aggrecan purified from 4 M guanidine hydrochloride extract were analyzed using HPLCs and atomic force microscopy with bovine tracheal cartilage aggrecan as a control. The estimated numbers of sulfates per disaccharide unit of chondroitin sulfate chains of salmon and bovine aggrecans were similar (approximately 0.85). However, the disaccharide composition showed a higher proportion of chondroitin 6-sulfate units in salmon aggrecan, 60%, compared to 40% in bovine. Gel filtration HPLC and monosaccharide analysis showed the salmon aggrecan had a lower number (approximately one-third), but 1.5-3.3 times longer chondroitin sulfate chains than the bovine aggrecan. Atomic force microscopic molecular images of aggrecan supported the images predicted by biochemical analyses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 103, 15 March 2014, Pages 538-549
نویسندگان
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