Glycosylation of Nα-lauryl-O-(β-d-xylopyranosyl)-l-serinamide as a saccharide primer in cells

► Xyl-Ser-C12 saccharide primer was synthesized to obtain glycosaminoglycan oligosaccharides. ► Glycosylated products of Xyl-Ser-C12 from cells were analyzed by LC-MS/MS and glycosidase digestion. ► Xyl-Ser-C12 exhibited good acceptor activity for the glycosylation of glycosaminoglycan-type oligosaccharides.