Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12

► Metabolic engineering of E. coli K-12 allows intracellular production of heparosan. ► The intracellular location of heparosan allows subsequent enzymatic modifications. ► Co-expression of K5-lyase allows production of heparosan oligosaccharides.