Templated polycondensation of aminopropyltrimethoxysilane on DNA

The polycondensation of a silane derivative such as aminopropyltrimethoxysilane (ATMS) in the presence of DNA was investigated in an organic solvent for the first time. Here, NMR observation showed that the hydrolysis step of ATMS before polycondensation was faster when the reaction was carried out in presence of double stranded DNA (146 bp) in chloroform. In order to test the specificity of this enhancement effect, the influence of the different components of DNA on ATMS hydrolysis and/or polycondensation was then investigated using different bases, nucleosides and nucleotides. The results showed that the kinetics of ATMS hydrolysis was affected by the type of nucleotide units used, the faster hydrolysis reaction rate being observed with molecules containing adenosine group, and that in the absence of water the amino group of deoxyadenosine units can react with ATMS methoxy groups.