Maximum entropy analysis of data simulations and practical aspects of time-resolved fluorescence measurements in the study of molecular interactions

- Advantage of prior knowledge option in the quantified MEM software of data analysis.
- Decays overlap should be taken into account in FLIM data analysis.
- Usefullness of simulated data with non-random noise for accuracy limit estimation.
- Increasing counting yields more accurate rotational correlation time determination.
- Too fast acquisition of excitation profile causes time shift and distortion.