Improved chromatography reveals multiple new bacteriohopanepolyol isomers in marine sediments

Bacteriohopanepolyols (BHPs) are characterized by a large structural diversity, although methodological constraints have limited investigations of the occurrence of isomers among composite BHPs in environmental samples. Here, we describe a novel chromatography method that uses three serial Phenomenex Kinetex C18 columns to successfully resolve new structural isomers of BHPs commonly observed in marine sediment samples. The investigated samples consistently contain a high diversity of BHP isomers, but their relative abundances differ significantly between samples. These differences in relative abundance have potential to reflect different environmental conditions such as depositional setting or redox conditions. The improved BHP resolution and baseline separation of the new method is promising for accurate quantification and future environmental proxy development and compound-specific isotope work.