کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7831967 | 1503281 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation and Characterization of a Novel Magnetic Nano-Gene Vector
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preparation and Characterization of a Novel Magnetic Nano-Gene Vector Preparation and Characterization of a Novel Magnetic Nano-Gene Vector](/preview/png/7831967.png)
چکیده انگلیسی
In recent times, as nonviral gene vectors, chitosan and its derivations have attracted many researchers' attentions. The preparation of a novel gene vector-magnetic nanoparticle coated with a novel polymer-lysine modified chitosan (CTS-lys) is described in this article. Initially, the correlation conditions of preparing CTS-lys were optimized, and it was demonstrated that lots of amines of chitosan were modified by lysine, which was indicated by infrared spectroscopy (IR) and proton nuclear magnetic resonance (1H-NMR). Subsequently, through the coprecipitation method, the lysine modified chitosan magnetic nanospheres (CTS-lys-MNPs) were attained. The characterizations of nanospheres were measured by transmission electron microscopy (TEM), dynamic laser light scattering, vibrating samples magnetometer (VSM), and X-ray diffraction (XRD). The cytotoxicity of CTS-lys-MNPs was also investigated with U293 cells. The results indicated that the average size of nanospheres was about 100 nm, and they had narrower size distribution, good superparamagnetic property, and perfect crystallinity, and they also retained low toxicity. Additionally, the combination of DNA and CTS-lys-MNPs was observed via agarose gel electrophoresis, and the ability of complexes of CTS-lys-MNPs and DNA crossing blood-brain barrier (BBB) in rats was studied by single photon emission computed tomography (SPECT). The results showed that the gene vector was a superior material, which had the ability of targeting and avoiding the caption of BBB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Physico-Chimica Sinica - Volume 23, Issue 12, December 2007, Pages 1857-1863
Journal: Acta Physico-Chimica Sinica - Volume 23, Issue 12, December 2007, Pages 1857-1863
نویسندگان
Lijuan Hao, Shuangyan Li, Lei Han, Jie Huang, Jin Chang,