کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7840157 | 1505865 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphorescent detection of DNA- drug interaction based on emission quenching of ZnS quantum dots via photoinduced electron transfer
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel room temperature phosphorescence (RTP) sensor consisting of quantum dots (QDs)-drug nanohybrid was used for the investigation of interaction between epirubicin (EPI) and double-stranded deoxyribonucleic acid (ds-DNA). The method based on the quenching effect of EPI on the phosphorescence emission of Mn-doped ZnS QDs via photoinduced electron-transfer (PIET) mechanism. Adsorption of EPI to the QDs surface caused quenching of RTP emission of QDs via photoinduced electron-transfer process. Whereas, with the addition of DNA provided the restoration of emission due to removing of EPI from the surface. The quantum dots were synthesized in an aqueous medium and characterized. The diameter of prepared QDs were about 3.5â¯nm, spherical, and uniform size. The quenching mechanism of QDs by EPI is not only collisional but also static. The static and dynamic quenching constants were found as 5.36 à 105 Mâ1 and 3.19 à 104 Mâ1, respectively. In addition to this method, fluorescence and absorption spectrometric methods were used to evaluate DNA/drug interaction and calculate the binding constant (K), which was 3.83 à 105 Mâ1. Proposed method has advantages such as simplicity and avoids interferences from autofluorescence and scattering light.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 197, May 2018, Pages 112-118
Journal: Journal of Luminescence - Volume 197, May 2018, Pages 112-118
نویسندگان
Ilkay Ocak, Hayriye Eda Satana Kara,