کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7842002 | 1506507 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Micellization and aggregation behavior of crocin with bovine serum albumin: A biophysical study
ترجمه فارسی عنوان
رفتار مایکولیزاسیون و تجمع کروکین با آلبومین سرم گاوی: یک مطالعه بیوفیزیکی
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
چکیده انگلیسی
The interaction of bovine serum albumin (BSA) with a naturally occurring surfactant (trans-crocetin di-(β-D-gentiobiosyl) ester, crocin) was studied under biophysical conditions by using various techniques such as surface tensiometry, UV-visible spectrophotometery and fluorescence. Upon the addition of a small amount of BSA, the λmax of a crocin dispersion in water shifted to lower wavelengths, indicating either a ground state complex formation between BSA and crocin or solubilization of BSA into the micelles of crocin. The critical micelle concentration (cmc) of crocin decreases in the presence of increasing BSA concentrations. The complex formation constant (KC), surface pressure Ïcmc, maximum surface excess (Îmax), the minimum surface area per molecule (Amin), enthalpy (ÎH0), entropy (ÎS0), and Gibbs free energy change (ÎG0) were calculated for the BSA-crocin interactions. BSA fluorescence was quenched in the presence of crocin. The fluorescence data were used for determining the apparent association constant (Kapp), the Stern-Volmer constant (KSV), the bimolecular rate constant of the quenching process (kq) and the thermodynamic parameters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Liquids - Volume 264, 15 August 2018, Pages 17-23
Journal: Journal of Molecular Liquids - Volume 264, 15 August 2018, Pages 17-23
نویسندگان
Zaheer Khan, Shaeel Ahmad Al-Thabaiti,