کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7986496 1515136 2016 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytopathology of the trachea of Bombyx mori (Lepidoptera: Bombycidae) to Bombyx mori nucleopolyhedrovirus
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد دانش مواد (عمومی)
پیش نمایش صفحه اول مقاله
Cytopathology of the trachea of Bombyx mori (Lepidoptera: Bombycidae) to Bombyx mori nucleopolyhedrovirus
چکیده انگلیسی
Bombyx mori is a holometabolous insect found only in germplasm banks and morphological data related of resistance and susceptibility to diseases is important when selecting hybrids for commercial and scientific interest. This study analyzed the cytopathology of B. mori trachea to BmNPV, isolated geographically in Paraná state, Brazil. Fifth instar larvae were divided into two groups, control and inoculated; the viral suspension used was 2.4 × 107 polyhedral occlusion bodies/mL. From the second to the ninth day post-inoculation, segments of silkworm organs, containing the trachea, were processed for transmission electron microscopy. Analyses of fresh hemolymph were also performed to verify the susceptibility of the hemocytes. Signs of infection were initially detected in the hemocytes and in the tracheal cells on the second and fourth post-inoculation days, respectively. The cytopathology of the trachea showed all stages of the viral cycle, which was the same as in other tissues. Virions were detected in the basal lamina, which showed structural disorganization. So, the infection time of the hemocytes prior to trachea and the presence of virus in basal lamina, suggests that the trachea was a secondary target, infected by budded virus coming from the hemolymph.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 80, January 2016, Pages 39-44
نویسندگان
, , , ,