کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8047227 | 1519088 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Palygorskite-poly(o-phenylenediamine) nanocomposite: An enhanced electrochemical platform for glucose biosensing
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
ژئوشیمی و پترولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Palygorskite-poly(o-phenylenediamine) nanocomposite: An enhanced electrochemical platform for glucose biosensing Palygorskite-poly(o-phenylenediamine) nanocomposite: An enhanced electrochemical platform for glucose biosensing](/preview/png/8047227.png)
چکیده انگلیسی
Palygorskite (Pal) may be a promising material for enzyme immobilization due to its large surface, high biocompatibility and stability. This attractive material combined with a conducting polymer, poly(o-phenylenediamine), was exploited as a platform for the immobilization of glucose oxidase (GOD) using glutaraldehyde as crosslinker, and thus a novel glucose biosensor was obtained. The results of electrochemical impedance spectroscopy (EIS) and SEM indicated the successful entrapment of GOD in the clay polymer nanocomposite (CPN) film. Amperometric detection of glucose was performed by holding the potential at the CPN electrode at 0.6Â V for the oxidation of H2O2 generated in the enzymatic reaction. The apparent Michaelis-Menten constant (KMapp) was calculated to be 5.25Â mM, which is close to that of the free enzyme. The proposed biosensor exhibited a wide linear range, a low detection limit, a good reproducibility and accepted stability in the determination of glucose, providing a biocompatible platform for glucose biosensing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Clay Science - Volume 86, December 2013, Pages 59-63
Journal: Applied Clay Science - Volume 86, December 2013, Pages 59-63
نویسندگان
Shiping Luo, Yu Chen, Min Zhou, Chao Yao, Haitao Xi, Yong Kong, Linhong Deng,