کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8086939 1521815 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Profiling of complex lipids in marine microalgae by UHPLC/tandem mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
Profiling of complex lipids in marine microalgae by UHPLC/tandem mass spectrometry
چکیده انگلیسی
Microalgal lipids are of great interest for their potential as food and feed ingredients, nutraceutical and cosmetic components, and as source of energy. Glycerol-based lipids can constitute up to 50% or more of the biomass of microalgae. How synthesis of these products takes place is still poorly explored, although remarkable differences with plants and among microalgal species have already emerged. These differences attain to both lipid class and fatty acyl composition, which could be modulated by specific growth conditions. Here we present a new method based on UHPLC coupled to high resolution tandem mass spectrometry for targeted analysis of the main lipid classes, i.e., glycolipids, phospholipids and triacylglycerols, applied to five marine microalgae exhibiting a great diversity in lipid composition. The LC/MS/MS approach is integrated with a recently proposed MTBE-based protocol for lipid extraction and the entire workflow is of general application to lipid profiling of cell extracts from different sources.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 17, July 2016, Pages 348-358
نویسندگان
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