کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8087148 | 1521816 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae
ترجمه فارسی عنوان
میکروسکوپ رامان و فلورسانس ساختارهای انرژی انتقال و ذخیره انرژی در میکروالگوها
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
چکیده انگلیسی
Chlorophylls and carotenoids are core components of photosynthetic energy transduction in algal cells, whereas starch, lipids, and polyphosphates represent energy reserves. All these biomolecules exhibit characteristic molecular vibrations that were sensed and localized in individual cells of Desmodesmus quadricauda and Chlorella vulgaris by confocal Raman microscopy. In the same cells, fluorescence of chlorophylls and of lipid bodies stained with Nile Red was mapped using the same instrument. In the first of the three consecutive scans, a low-power and short-exposure excitation by a green 532Â nm laser was used to map chlorophyll fluorescence. In the second scan, the full power of a 785Â nm laser was used for Raman mapping of carotenoids. The third scan was performed again with the 532Â nm laser to map non-fluorescent biomolecules such as lipids, proteins, starch, and polyphosphates. Before the third scan, chlorophyll fluorescence was suppressed by photobleaching a wide area using a strongly defocused 532Â nm laser beam. Raman microscopy was used for the first time to localize polyphosphate granules within a single algal cell in the context of other relevant bio-energetic structures. The new information represents an opportunity for algal cell phenotyping.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Algal Research - Volume 16, June 2016, Pages 224-232
Journal: Algal Research - Volume 16, June 2016, Pages 224-232
نویسندگان
Å árka MoudÅÃková, Peter MojzeÅ¡, Vilém Zachleder, Christian Pfaff, Dominik Behrendt, Ladislav Nedbal,