کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8135019 1523515 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of HPLC-Orbitrap method for identification of N-bearing molecules in complex organic material relevant to planetary environments
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات علوم فضا و نجوم
پیش نمایش صفحه اول مقاله
Development of HPLC-Orbitrap method for identification of N-bearing molecules in complex organic material relevant to planetary environments
چکیده انگلیسی
Although the Cassini Spacecraft and the Huygens lander provided vast information about Titan atmospheric chemistry and the formation of its aerosols, the exact composition of these aerosols still remains unknown. A fruitful proxy to investigate these aerosols is the use of laboratory experiments that allow producing and studying analogs of Titan aerosol, the so-called tholins. Even when produced in the laboratory, unveiling the exact composition of the aerosol remains problematic due to the high complexity of the material. Numerous advances have been recently made using high-resolution mass spectrometry (HRMS) (Pernot et al. [2010] Anal. Chem. 82, 1371; Somogyi et al. [2012] Int. J. Mass Spectrom. 316-318, 157-163; Gautier et al. [2014] Earth Planet. Sci. Lett. 404, 33-42) that allowed the separation of isobaric compounds and a robust identification of chemical species composing tholins regarding their molecular formulae. Nevertheless isomeric species cannot be resolved by a simple mass measurement. We propose here an analysis of tholins by high performance liquid chromatography (HPLC) coupled to HRMS to unveil this isomeric ambiguity for some of the major tholins compounds. By comparing chromatograms obtained when analyzing tholins and chemical standards, we strictly identified seven molecules in our tholins samples: melamine, cyanoguanidine, 6-methyl-1,3,5-triazine-2,4-diamine, 2,4,6-triaminopyrimidine, 3-amino-1,2,4-triazole, 3,5-Dimethyl-1,2,4-triazole and 2,4-diamino-1,3,5-triazine. Several molecules, including hexamethylenetriamine (HMT) were not present at detectable levels in our sample. The use for the first time of a coupled HPLC-HRMS technique applied to tholins study demonstrated the interest of such a technique compared to single high-resolution mass spectrometry for the study of tholins composition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Icarus - Volume 275, 1 September 2016, Pages 259-266
نویسندگان
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