Estimation of collagen presence in dry bone using combined X-ray and neutron radiography

Thermal neutron radiography and X-ray radiography are characterised by different penetration depths in various materials, for example in collagen and hydroxyapatite, two major components of bone. Neutron radiography penetrates hydroxyapatite easier than collagen and, conversely, in X-ray radiography attenuation is higher in hydroxyapatite than in collagen. This effect allows estimation of collagen presence in dry bone. In our study we show that differences between the two imaging methods are sufficient to produce significant results when bone areas with higher and lower content of collagen are being compared.