کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8268200 | 1534953 | 2016 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pre-treatment of rats with ad-hepcidin prevents iron-induced oxidative stress in the brain
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کلمات کلیدی
Ferroportin 1BMECsFpn1NTBITfR1DMT1GFAASROS - ROStransferrin-bound iron - آهن سرم انتقالینnon-transferrin-bound iron - آهن غیر انتقال سرمStriatum - استریاتومAlzheimer’s disease - بیماری آلزایمرParkinson’s disease - بیماری پارکینسونsubstantia nigra - توده سیاهBBB - سد خونی مغزیbrain microvascular endothelial cells - سلولهای اندوتلیالی مغز استخوان مغزCortex - قشرBrain–blood barrier - مانع مغز و خونDivalent metal transporter 1 - متال فلچر 1Hippocampus - هیپوکامپ Reactive oxygen species (ROS) - گونه های اکسیژن واکنشی (ROS)Reactive oxygen species - گونههای فعال اکسیژنtransferrin receptor-1 - گیرنده پروتئین 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Our recent investigation showed that hepcidin can reduce iron in the brain of iron-overloaded rat by down-regulating iron-transport proteins. It has also been demonstrated that iron is a major generator of reactive oxygen species. We therefore hypothesized that hepcidin could prevent iron accumulation and thus reduce iron-mediated oxidative stress in iron-overloaded rats. To test this hypothesis, we investigated the effects of pre-treatment of rats with recombinant-hepcidin-adenovirus (ad-hepcidin) on the contents of iron, dichlorofluorescein and 8-isoprostane in the brain. Hepcidin expression was detected by real-time PCR and immunofluorescence analysis. Iron contents were measured using Perl's staining as well as graphite furnace atomic absorption spectrophotometry. Dichlorofluorescein and 8-isoprostane were determined using a fluorescence spectrophotometer and an ELISA kit, respectively. We found that hepcidin contents in the cortex, hippocampus, striatum and substantia nigra of rats treated with ad-hepcidin are 3.50, 2.98, 2.93 and 4.07 fold of those of the control rats respectively. Also, we demonstrated that the increased iron as well as dichlorofluorescein and 8-isoprostane levels in all four brain regions, induced by injection of iron dextran, could be effectively prevented by pre-treatment of the rats with ad-hepcidin. We concluded that pre-treatment with ad-hepcidin could increase hepcidin expression and prevent the increase in iron and reduce reactive oxygen species in the brain of iron-overloaded rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 90, January 2016, Pages 126-132
Journal: Free Radical Biology and Medicine - Volume 90, January 2016, Pages 126-132
نویسندگان
Jing Gong, Fang Du, Zhong Ming Qian, Qian Qian Luo, Yuan Sheng, Wing-ho Yung, Yan Xin Xu, Ya Ke,