کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8268262 | 1534953 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Momordin Ic couples apoptosis with autophagy in human hepatoblastoma cancer cells by reactive oxygen species (ROS)-mediated PI3K/Akt and MAPK signaling pathways
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کلمات کلیدی
Momordin Ichuman hepatoblastomaMMPPARPJnk3-MAMDCRAPHepG2DCFH-DANAC2′,7′-dichlorofluorescin diacetate - 2 '، 7'-dichlorofluorescin diacetate3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromide3-methyladenine - 3-متیل آدنینc-Jun N-terminal kinase - C-Jun N-terminal kinaseERK1/2 - ERK1 / 2MAPK - MAPKMTT - MTTPI3K/AKT - PI3K / AKTROS - ROSAutophagy - اتوفاژیApoptosis - خزان یاختهایRapamycin - راپامایسینHepG2 cells - سلولهای HepG2Monodansylcadaverine - مونودانسیل کاداورینCrosstalk - هم شنوی، کراس تاک، نشت سیگنالMitochondrial membrane potential - پتانسیل غشای میتوکندریmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenpoly (ADP-ribose) polymerase - پلی (ADP-ribose) پلیمرازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Momordin Ic is a principal saponin constituent of Fructus Kochiae, which acts as an edible and pharmaceutical product more than 2000 years in China. Our previous research found momordin Ic induced apoptosis by PI3K/Akt and MAPK signaling pathways in HepG2 cells. While the role of autophagy in momordin Ic induced cell death has not been discussed, and the connection between the apoptosis and autophagy is not clear yet. In this work, we reported momordin Ic promoted the formation of autophagic vacuole and expression of Beclin 1 and LC-3 in a dose- and time-dependent manner. Compared with momordin Ic treatment alone, the autophagy inhibitor 3-methyladenine (3-MA) also can inhibit apoptosis, while autophagy activator rapamycin (RAP) has the opposite effect, and the apoptosis inhibitor ZVAD-fmk also inhibited autophagy induced by momordin Ic. Momordin Ic simultaneously induces autophagy and apoptosis by suppressing the ROS-mediated PI3K/Akt and activating the ROS-related JNK and P38 pathways. Additionally, momordin Ic induces apoptosis by suppressing PI3K/Akt-dependent NF-κB pathways and promotes autophagy by ROS-mediated Erk signaling pathway. Those results suggest that momordin Ic has great potential as a nutritional preventive strategy in cancer therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 90, January 2016, Pages 230-242
Journal: Free Radical Biology and Medicine - Volume 90, January 2016, Pages 230-242
نویسندگان
Yashi Mi, Chunxia Xiao, Qingwei Du, Wanqiang Wu, Guoyuan Qi, Xuebo Liu,