کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8269710 | 1534965 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetic and structural characterization of a typical two-cysteine peroxiredoxin from Leptospira interrogans exhibiting redox sensitivity
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کلمات کلیدی
DTTTrxTrxRtert-butylhydroperoxideahpCLeptospira interrogans2-hydroxyethyl disulfideResolving Cyst-BOOH - T-BOOHthioredoxin reductase - تریودوکسین ردوکتازthioredoxin - تیرودوکسینdithiothreitol - دیتیوتریتولFree radicals - رادیکال آزادHEDs - زیباییThioredoxin system - سیستم تیورودوکسینPeroxide - پراکسید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Kinetic and structural characterization of a typical two-cysteine peroxiredoxin from Leptospira interrogans exhibiting redox sensitivity Kinetic and structural characterization of a typical two-cysteine peroxiredoxin from Leptospira interrogans exhibiting redox sensitivity](/preview/png/8269710.png)
چکیده انگلیسی
Little is known about the mechanisms by which Leptospira interrogans, the causative agent of leptospirosis, copes with oxidative stress at the time it establishes persistent infection within its human host. We report the molecular cloning of a gene encoding a 2-Cys peroxiredoxin (LinAhpC) from this bacterium. After bioinformatic analysis we found that LinAhpC contains the characteristic GGIG and YF motifs present in peroxiredoxins that are sensitive to overoxidation (mainly eukaryotic proteins). These motifs are absent in insensitive prokaryotic enzymes. Recombinant LinAhpC showed activity as a thioredoxin peroxidase with sensitivity to overoxidation by H2O2 (Chyp 1% ~30 µM at pH 7.0 and 30 °C). So far, Anabaena 2-Cys peroxiredoxin, Helicobacter pylori AhpC, and LinAhpC are the only prokaryotic enzymes studied with these characteristics. The properties determined for LinAhpC suggest that the protein could be critical for the antioxidant defense capacity in L. interrogans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 77, December 2014, Pages 30-40
Journal: Free Radical Biology and Medicine - Volume 77, December 2014, Pages 30-40
نویسندگان
Diego G. Arias, Anahà Reinoso, Natalia Sasoni, MatÃas D. Hartman, Alberto A. Iglesias, Sergio A. Guerrero,