کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8269894 | 1534965 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanisms and kinetic profiles of superoxide-stimulated nitrosative processes in cells using a diaminofluorescein probe
ترجمه فارسی عنوان
مکانیسم ها و پروفیل های جنبشی فرایندهای نیتروژنیک تحریک شده توسط سوپراکسید در سلول ها با استفاده از یک پروب دیامینی فلوئورسین
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کلمات کلیدی
2,3-dimethoxy-1,4-naphthoquinoneLPSPEG-SODMnTE-2-PyPCarbonate anion radicalPROLI/NOFCNDAFMnTBAPHbO2BSODTPAOxyhemoglobinDMNQO2− - O2-Nitrosative stress - استرس نیتروژنbuthionine sulfoximine - بوته یون سولفسیمیمDiethylenetriaminepentaacetic acid - دی اتیلنتریمین پنتا اسیدهای اسیدdiaminofluorescein - دیامین فلوئورسینSuperoxide anion radical - رادیکال آنیون سوپراکسیدSOD - سدSuperoxide - سوپر اکسیدNitrosylation - نیتروسیلهNitrosation - نیتروژنNO2 - نیتروژن دیاکسیدNitric oxide - نیتریک اکسیدPeroxynitrite - پروکسی نیتریت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
In this study, we examined the mechanisms and kinetic profiles of intracellular nitrosative processes using diaminofluorescein (DAF-2) as a target in RAW 264.7 cells. The intracellular formation of the fluorescent, nitrosated product diaminofluorescein triazol (DAFT) from both endogenous and exogenous nitric oxide (NO) was prevented by deoxygenation and by cell membrane-permeable superoxide (O2â) scavengers but not by extracellular bovine Cu,Zn-SOD. In addition, the DAFT formation rate decreased in the presence of cell membrane-permeable Mn porphyrins that are known to scavenge peroxynitrite (ONOOâ) but was enhanced by HCO3â/CO2. Together, these results indicate that nitrosative processes in RAW 264.7 cells depend on endogenous intracellular O2â and are stimulated by ONOOâ/CO2-derived radical oxidants. The N2O3 scavenger sodium azide (NaN3) only partially attenuated the DAFT formation rate and only with high NO (>120Â nM), suggesting that DAFT formation occurs by nitrosation (azide-susceptible DAFT formation) and predominantly by oxidative nitrosylation (azide-resistant DAFT formation). Interestingly, the DAFT formation rate increased linearly with NO concentrations of up to 120-140Â nM but thereafter underwent a sharp transition and became insensitive to NO. This behavior indicates the sudden exhaustion of an endogenous cell substrate that reacts rapidly with NO and induces nitrosative processes, consistent with the involvement of intracellular O2â. On the other hand, intracellular DAFT formation stimulated by a fixed flux of xanthine oxidase-derived extracellular O2â that also occurs by nitrosation and oxidative nitrosylation increased, peaked, and then decreased with increasing NO, as previously observed. Thus, our findings complementarily show that intra- and extracellular O2â-dependent nitrosative processes occurring by the same chemical mechanisms do not necessarily depend on NO concentration and exhibit different unusual kinetic profiles with NO dynamics, depending on the biological compartment in which NO and O2â interact.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 77, December 2014, Pages 270-280
Journal: Free Radical Biology and Medicine - Volume 77, December 2014, Pages 270-280
نویسندگان
Fernando Cruvinel Damasceno, Rômulo Rodrigues Facci, Thalita Marques da Silva, José Carlos Jr.,