کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8269994 | 1534965 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Survival signaling elicited by 27-hydroxycholesterol through the combined modulation of cellular redox state and ERK/Akt phosphorylation
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کلمات کلیدی
27-HydroxylaseHRPBcl-2ERKCYP27A1LXRM-CSFJC-17-Ketocholesterol - 7-کتو کلسترولdihydroethidium - دی هیدروتیدیمB-cell lymphoma 2 - لنفوم سلول B 2macrophage colony-stimulating factor - ماکروفاژ عامل کلونی تحریک کنندهMEK - مجاهدین خلقDHE - وHorseradish peroxidase - پراکسیداز هوررادیشliver X receptor - کبد X گیرندهextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The oxysterol 27-hydroxycholesterol (27-OH) is increasingly considered to be involved in a variety of pathophysiological processes, having been shown to modulate cell proliferation and metabolism, and also to exert proinflammatory and proapoptotic effects. This study aimed to elucidate the molecular pathways whereby 27-OH may generate survival signals in cells of the macrophage lineage, and to clarify whether its known prooxidant effect is involved in that process. A net up-regulation of survival signaling, involving the extracellular signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K)/Akt phosphorylation pathways, was observed in U937 promonocytic cells cultivated over time in the presence of a low micromolar concentration of the oxysterol. Interestingly, the up-regulation of both kinases was shown to be closely dependent on an early 27-OH-induced intracellular increase of reactive oxygen species (ROS). In turn, stimulation of ERK and PI3K/Akt both significantly quenched ROS steady state and markedly phosphorylated Bad, thereby determining a marked delay of the oxysterol׳s proapoptotic action. The 27-OH-induced survival pathways thus appear to be redox modulated and, if they occur within or nearby inflammatory cells during progression of chronic diseases such as cancer and atherosclerosis, they could significantly impact the growth and evolution of such diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 77, December 2014, Pages 376-385
Journal: Free Radical Biology and Medicine - Volume 77, December 2014, Pages 376-385
نویسندگان
Beyza Vurusaner, Paola Gamba, Gabriella Testa, Simona Gargiulo, Fiorella Biasi, Chiara Zerbinati, Luigi Iuliano, Gabriella Leonarduzzi, Huveyda Basaga, Giuseppe Poli,