کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8271679 | 1534984 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impact of hydrogen peroxide-driven Fenton reaction on mouse oocyte quality
ترجمه فارسی عنوان
تاثیر واکنش فنتون بر پراکسید هیدروژن بر کیفیت تخم مرغ ماوس
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کلمات کلیدی
رادیکال هیدروکسیل، آب اکسیژنه، آهن آزاد واکنش فنتون، گونه های اکسیژن واکنش پذیر، التهاب لگن، آندومتریوز، پیری تخمک استرس اکسیداتیو، میلوپروکسیداز، پروتئین هام رادیکال های آزاد،
Hydrogen peroxide - آب اکسیژنهFree iron - آهن آزادEndometriosis - اندومتریوزOxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادHydroxyl radical - رادیکال هیدروکسیلmyeloperoxidase - میلوپراکسیداز Fenton reaction - واکنش فنتونHeme protein - پروتئین هامoocyte aging - پیری تخمکReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
Here we show that hydroxyl radical (
- OH) generated through the Fenton reaction alters metaphase-II mouse oocyte microtubules (MT) and chromosomal alignment (CH). Metaphase-II mouse oocytes, obtained commercially, were grouped as follows: control, hydrogen peroxide (H2O2), Fe(II), and combined (Fe(II) +H2O2) treatments. After 7-10 min of incubation at 37 °C, MT and CH were evaluated on fixed and stained oocytes and scored by two blinded observers. Pearson Ï2 test and Fisher exact test were used to compare outcomes between controls and treated groups and also among the treated groups. Our results showed that poor scores for MT and CH increased significantly in oocytes treated with a combination of H2O2 and Fe(II) (p<0.001); oocytes treated with H2O2 alone or Fe(II) alone showed no or few changes compared to control. Comparison of oocyte groups that received increasing concentrations of H2O2 and a fixed amount of Fe(II) showed that 70-80% demonstrated poor scores in both MT and CH when pretreated with 5 μM H2O2, and this increased up to 90-100% when treated with 10-20 μM H2O2. Hydroxyl radical generated by H2O2-driven Fenton reaction deteriorates the metaphase-II mouse oocyte spindle and CH alignment, which is thought to be a potential cause of poor oocyte quality. Thus, free iron and/or ROS scavengers could attenuate the
- OH-mediated spindle and chromosomal damage, thereby serving as a possible approach for further examination as a therapeutic option in inflammatory states.
- OH) generated through the Fenton reaction alters metaphase-II mouse oocyte microtubules (MT) and chromosomal alignment (CH). Metaphase-II mouse oocytes, obtained commercially, were grouped as follows: control, hydrogen peroxide (H2O2), Fe(II), and combined (Fe(II) +H2O2) treatments. After 7-10 min of incubation at 37 °C, MT and CH were evaluated on fixed and stained oocytes and scored by two blinded observers. Pearson Ï2 test and Fisher exact test were used to compare outcomes between controls and treated groups and also among the treated groups. Our results showed that poor scores for MT and CH increased significantly in oocytes treated with a combination of H2O2 and Fe(II) (p<0.001); oocytes treated with H2O2 alone or Fe(II) alone showed no or few changes compared to control. Comparison of oocyte groups that received increasing concentrations of H2O2 and a fixed amount of Fe(II) showed that 70-80% demonstrated poor scores in both MT and CH when pretreated with 5 μM H2O2, and this increased up to 90-100% when treated with 10-20 μM H2O2. Hydroxyl radical generated by H2O2-driven Fenton reaction deteriorates the metaphase-II mouse oocyte spindle and CH alignment, which is thought to be a potential cause of poor oocyte quality. Thus, free iron and/or ROS scavengers could attenuate the
- OH-mediated spindle and chromosomal damage, thereby serving as a possible approach for further examination as a therapeutic option in inflammatory states.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 58, May 2013, Pages 154-159
Journal: Free Radical Biology and Medicine - Volume 58, May 2013, Pages 154-159
نویسندگان
Faten Shaeib, Jashoman Banerjee, Dhiman Maitra, Michael P. Diamond, Husam M. Abu-Soud,